Purification and on-column activation of a recombinant histidine-tagged pro-transglutaminase after soluble expression in Escherichia coli.
نویسندگان
چکیده
Microbial transglutaminase (MTG) is widely used as a protein crosslinking enzyme. Pro-transglutaminase from Streptomyces mobaraensis was expressed in Escherichia coli as a fusion protein carrying a C-terminal histidine tag (pro-MTG-His(6)) under high-density culture. A new method of on-column activation was designed for production. According to SDS-PAGE, 88.9% of pro-MTG-His(6) was transferred to mature MTG-His(6) with storage stabilization.
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 73 11 شماره
صفحات -
تاریخ انتشار 2009